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微信小章| 产品名称： | pHDR5A |
|---|---|
| 商品货号： | TS156852 |
| Designations： | pHDR5A |
| GenBank Number： | M14758 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 4.199999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.379999995231628 Tissue: carcinoma KB-C2.5 cell line Gene product: P glycoprotein 1/multiple drug resistance 1 MDR1 |
| Insert Size (kb)： | 1.380 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | The insert contains the following restriction sites (at nucleotide positions according to the published sequence): PvuII--1314, XmnI--1560, PvuII--1921, HindIII--2041; StuI--2168; XmnI--2292. Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.4; HindIII--3.3, 0.9; BamHI--4.3; SacI--4.3. The insert includes nucleotides 1178 to 2561 of the cDNA. This probe detects a mRNA of approximately 4.4-4.5 kb. The insert is oriented such that transcription from the T7 promoter gives the sense strand. |
| References： | Galski H, et al. Expression of a human multidrug resistance cDNA (MDR1) in the bone marrow of transgenic mice: resistance to daunomycin-induced leukopenia. Mol. Cell. Biol. 9: 4357-4363, 1989. PubMed: 2573831 Chen CJ, et al. Internal duplication and homology with bacterial transport proteins in the mdr1 (P-glycoprotein) gene from multidrug-resistant human cells. Cell 47: 381-389, 1986. PubMed: 2876781 Ueda K, et al. The human multidrug resistance (mdr1) gene: cDNA cloning and transcription initiation. J. Biol. Chem. 262: 505-508, 1987. PubMed: 3027054 Michael M Gottesman, personal communication |