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| 产品名称： | pVH-3 |
|---|---|
| 商品货号： | TS156922 |
| Designations： | pVH-3 |
| Species： | Potato spindle tuber viroid |
| Depositors： | RA Owens |
| Vector： | Construct size (kb): 4.699999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.3400000035762787 |
| Insert Size (kb)： | 0.340 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains internal sites for AvaII, AluI, BamHI and SmaI. Restriction digests of the clone give the following sizes (kb): HaeIII--0.64, 0.54, 0.48, 0.38, plus smaller fragments; EcoRI--4.7; PstI--4.7; EcoRI/PstI--4.0, 0.74; EcoRI/PvuII--2.5, 2.4. Noninfectious unless released from plasmid by HaeIII digestion (and even then probably noninfectious). Full-length PSTV cDNA with HaeIII ends was cloned into the HindIII site of pBR322 using HindIII linkers. |
| Classification： | Pospiviroid |
| References： | Cress DE, et al. Construction of infectious potato spindle tuber viroid cDNA clones. Nucleic Acids Res. 11: 6821-6835, 1983. PubMed: 6314259 Robert A Owens, personal communication |