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| 产品名称： | pAAV/neo pAAV/SVneo |
|---|---|
| 商品货号： | TS156959 |
| Designations： | pAAV/neo pAAV/SVneo |
| Depositors： | Trustees of Princeton University, TE Shenk, Trustees of Princeton University |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.8000001907348630 Vector: pAAV/neo (phagemid) Promoters: Promoter for expression SV40 early Construction: pEMBL8+, neoR, AAV inverted terminal repeats Marker(s):G418R,ampR,neoR Construct size (kb): 6.800000190734863 Features: marker(s): ampR marker(s): neoR other: inverted terminal repeats (ITR) promoter for expression: SV40 early replicon: pMB1 terminator: SV40 small t splice site |
| Applications： | integrating vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--4.0, 2.3, 0.5; PvuII--3.6, 1.1, 0.78, 0.6, 0.35, 0.32; XbaI--4.0, 2.8. The cotransfected cells produce a recombinant virus which can be used to infect a human cell line, allowing integration of the cloned insert into the chromosome of the cell line. Integrating vector useful for creating recombinant adeno-associated virus when cotransfected into adenovirus infected cells containing the helper virus pAAV/Ad (ATCC 68066). The depositor recommends selection of a single colony and growth in LB + amp for less than 12 hours. Because of the AAV inverted repeats, the plasmid tends to rearrange if cultivated for longer periods of time. The neoR cassette can be excised using EcoRI/BamHI or XbaI allowing replacement by a cloned gene of interest. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Shenk TE, et al. Helper-free stocks of recombinant adeno-associated virus vectors. US Patent 5,436,146 dated Jul 25 1995 Samulski RJ, et al. Helper-free stocks of recombinant adeno-associated viruses: normal integration does not require viral gene expression. J. Virol. 63: 3822-3828, 1989. PubMed: 2547998 Thomas E Shenk, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |