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| 产品名称： | YEpFAT10 |
|---|---|
| 商品货号： | TS156967 |
| Designations： | YEpFAT10 |
| Depositors： | DJ Stillman, K Runge |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.7709999084472660 Vector: YEpFAT10 (plasmid) Promoters: Promoter for in vitro transcription T7 Construction: pVZ1 Marker(s):TRP1,ampR,LEU2-d Construct size (kb): 6.770999908447266 Features: marker(s): LEU2-d marker(s): TRP1 marker(s): ampR promoter: lac promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: 2 micron ori/LEU2-d replicon: pMB1 MCS: ApaI...SacII MCS: NruI...SphI |
| Applications： | YE-type (episomal) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI-- 4.2, 2.9; BamHI-- 7.0; SalI-- 7.0. Selection for TRP1 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200. Similarly, the NruI, BamHI, SalI and SphI are adjacent and can be used for directional cloning. The vector was constructed by Kurt Runge. One of 9 YE-type shuttle vectors (ATCC 87541, 87554, 87560 an d 87640 - 87645) that have the leu2-d gene, which is correlated with high copy number in S. cerevisiae, when grown in medium lacking leucine. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Conrad MN, et al. RAP1 protein interacts with yeast telomeres in vivo: overproduction alters telomere structure and decreases chromosome stability. Cell 63: 739-750, 1990. PubMed: 2225074 Runge KW, Zakian VA. Introduction of extra telomeric DNA sequences into Saccharomyces cerevisiae results in telomere elongation. Mol. Cell. Biol. 9: 1488-1497, 1989. PubMed: 2657397 |
| Shipped： | freeze-dried |
| Shipping Information： | xa0Freeze dried stock of Escherichia coli containing the plasmid. |