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| 产品名称： | pI304 |
|---|---|
| 商品货号： | TS156985 |
| Designations： | pI304 |
| Depositors： | KW Minton, HD Braymer |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 14.5000000000000000 Vector: pI304 (plasmid) Construction: pUE10, pKK232-8 Marker(s):ampR Construct size (kb): 14.5 Features: insert detection: CAT marker(s): ampR replicon: pMB1, pUE10 |
| Applications： | promoter-cloning vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--14.5; SalI--14.5; HindIII--9.4, 5.1. Promoter-cloning vector for Deinococcus radiodurans. Inserts with promoter function permit expression of chloramphenicol resistance. The order of the major features in this plasmid is: pUE10 replicon - cmlR - pMB1 ori - ampR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Masters CI, Minton KW. Promoter probe and shuttle plasmids for Deinococcus radiodurans. Plasmid 28: 258-261, 1992. PubMed: 1461940 |
| Shipped： | freeze-dried |