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| 产品名称： | lambdaMGU2 C. elegans cDNA library |
|---|---|
| 商品货号： | TS156987 |
| Designations： | lambdaMGU2 C. elegans cDNA library |
| Species： | Caenorhabditis elegans Maupas |
| Depositors： | IN Maruyama |
| Applications： | To prepare phagemid from lambdaMGU2, grow recombinants on a RecA- host expressing the Cre protein (E. coli 1046pCRE1, ATCC 77368) and select for ampicillin resistance.
Strain of C. elegans used for constructing this library is C. elegans N2 which is a wild type strain. |
| Vector： | Library information: Genome: Caenorhabditis elegans Strain: N2 Type of insert: cDNA Vector: lambdaMGU2 Insert size range (kb): 2-3 kb average Vector ends: Modification: BamHI Number of independent recombinants: 1.0 x 107 Titer: 5 x 107 pfu/mL Vector information: Vector: lambdaMGU2 Vector type: phage Intact vector size (kb): 41.7 Construction: lambda2690, pMGU Features: Insert detection: Spi+ Marker: ampR Replicon: lambda, pMB1, m13 |
| Related Products： | Component of: ATCC 77370 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Frozen bacteria-free phage lysate |
| Comments： | Strain of C. elegans used for constructing this library is C. elegans N2 which is a wild type strain.
Single-stranded DNA may be recovered from phagemid constructs using M13KO7 helper phages.
To prepare phagemid from lambdaMGU2, grow recombinants on a RecA- host expressing the Cre protein (E. coli 1046pCRE1, ATCC 77368) and select for ampicillin resistance. The pMGU product is 4.185 kb.
Inserts can be amplified using the following primers flanking the BamHI cloning site: upstream 5-AAGAGGCAGAACTGGCAG-3 and downstream 5-ATCGATGCATAGCGATTC-3.
The order of the major features in the cloning region of the lambda vector is: lambda J - SmaI - SalI - loxP - EcoRI - M13 ori - ampR - pMB1 ori - HindIII - 3gam/BamHI/5gam - XhoI - loxP - SalI - lambda N.
Efficiency of phagemid recovery is approximately 20%. Plasmid pCRE1 may be a low level contaminant, but is easily distinguished from pMGU DNA. |
| References： | Maruyama IN, Brenner S. A selective lambda phage cloning vector with automatic excision of the insert in a plasmid. Gene 120: 135-141, 1992. PubMed: 1327972 |