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| 产品名称： | pNK2882 |
|---|---|
| 商品货号： | TS157015 |
| Designations： | pNK2882 |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | N Kleckner |
| Vector： | Construct size (kb): 7.099999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 2.799999952316284 Gene product: transposase, Tn10, ats1 ats2 (ATS) |
| Insert Size (kb)： | 2.800 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--7.1; HindIII--4.6, 1.4; EcoRI--4.3, 2.8. Is compatible with plasmids derived from pBR322. Used with mini-Tn10 constructions for insertional mutagenesis of yeast and bacterial genes. Contains the ats1 ats2 transposase gene that permits relaxed insertion specificity (altered target specificity, ATS). Expression is regulated by the Ptac promoter, inducible by IPTG. The transposase segment extends from IS10R to the EcoRI site at nt 3140 of Tn10, with a deletion of nucleotides 1329-1942 to remove the transposase binding site. Transcription from the Ptac promoter is in a direction opposite to that of amp. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Kleckner N, et al. Uses of transposons with emphasis on Tn10. Methods Enzymol. 204: 139-180, 1991. PubMed: 1658561 |