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| 产品名称： | pREV-T3 |
|---|---|
| 商品货号： | TS157081 |
| Designations： | pREV-T3 |
| Species： | Avian reticuloendotheliosis virus (type C) |
| Depositors： | HM Temin |
| Vector： | Construct size (kb): 9.359999656677246 |
| Insert： | DNA: genomic Insert lengths(kb): 5.0 Tissue: DNA from chicken embryo fibroblasts Gene product: v-rel |
| Insert Size (kb)： | 5.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Shipped: xa0Freeze dried E. coli containing the plasmid |
| Comments： | MARKERS: Ap(r); Tc(r). Unintegrated circular REV-T DNA. Constructed by inserting permuted REV-T SalI fragment (one LTR) into SalI site of pBR322 MARKERS: Ap(r); Tc(r). A full-length clone that is infectious after SalI digestion and ligation. The insert is missing one LTR. The insert sequence starts at the SalI site preceding ATG for gag and continues beyond the LTR at the right. |
| References： | Chen IS, Temin HM. Substitution of 5 helper virus sequences into non-rel portion of reticuloendotheliosis virus strain T suppresses transformation of chicken spleen cells. Cell 31: 111-120, 1982. PubMed: 6297744 |