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| 产品名称： | pGEX-KG |
|---|---|
| 商品货号： | TS157103 |
| Designations： | pGEX-KG |
| Depositors： | JS Williams, JE Dixon |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 5.0000000000000000 DESCRIPTION OF VECTOR: Intact vector size: 5.000 Type of vector: plasmid Cloning sites: BamHI SmaI EcoRI XbaI NcoI SalI XhoI SacI HindIII Polylinker sites: EcoRI XbaI NcoI SalI XhoI SacI HindIII Construction: pGEX-2T Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR promoter: tac replicon: pMB1 repressor gene: lacIq Vector: pGEX-KG (plasmid) Promoters: Promoter tac Construction: pGEX-2T Marker(s):ampR Construct size (kb): 5.0 Features: marker(s): ampR promoter: tac replicon: pMB1 repressor gene: lacIq |
| Applications： | expression vector vector permitting construction of fusion proteins |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--5.0; BamHI--5.0; EcoRI--5.0; XbaI--5.0. Expression vector permitting production of a fusion protein. The glutathione S-transferase (GST) fusion protein can be purified by glutathione affinity chromatography, and the desired polypeptide released from the fusion product by thrombin. Constructed from pGEX-2T by inserting an oligonucleotide at the EcoRI site which encodes the glycine "kinker" and additional restriction sites to facilitate cloning in all reading frames. The order of the major features in this plasmid are: Ptac - GST - thrombin cleavage site - BamHI site - SmaI site - glycine "kinker" - multiple cloning site - ampR - pBR322 ori - lacIq. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Smith DB, Johnson KS. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67: 31-40, 1988. PubMed: 3047011 Guan KL, Dixon JE. Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase. Anal. Biochem. 192: 262-267, 1991. PubMed: 1852137 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |