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| 产品名称： | pYe(CEN3)30 |
|---|---|
| 商品货号： | TS157108 |
| Designations： | pYe(CEN3)30 |
| Depositors： | Amgen, Inc., BJ Ratzkin, Amgen, Inc. |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli JA228 |
| Vector Information： | Size (kb): 6.3000001907348630 Vector: pYe(CEN3)30 (plasmid) Construction: YRp7 Marker(s):TRP1,ampR Construct size (kb): 6.300000190734863 Features: marker(s): ampR, TRP1 replicon: pMB1, ARS1 centromere: CEN3 |
| Applications： | YC-type (centromeric) shuttle vector shuttle vector |
| Comments： | Contains unique BamHI, BglII, XbaI, NruI, and SalI sites, with XbaI and BglII inactivating TRP1. Restriction digests of the clone give the following sizes (kb): BamHI--6.3; BglII--6.3; SalI--6.3; EcoRI--4.6, 1.7. Demonstrates mitotic stability increased over YRp7; 60-90% of transformed S. cerevisiae J17 maintain the Trp+ phenotype after 20 generations. Constructed by inserting a 627 bp BamHI/Sau3AI fragment containing CEN3 into the BamHI site of YRp7. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Patel AC, Ratzkin BJ. Materials and methods for controlling plasmid copy number and stability. US Patent 4,935,350 dated Jun 19 1990 Fitzgerald-Hayes M, et al. Nucleotide sequence comparisons and functional analysis of yeast centromere DNAs. Cell 29: 235-244, 1982. PubMed: 7049398 Barry J Ratzkin, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |