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| 产品名称： | pM220 |
|---|---|
| 商品货号： | TS157136 |
| Designations： | pM220 |
| Depositors： | ZymoGenetics, Inc., JD Kelly, MJ Murray, ZymoGenetics, Inc. |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli RR1; K-12 RR1 |
| Vector Information： | Size (kb): 8.1999998092651370 Vector: pM220 (plasmid) Promoters: Promoter TPI Construction: TPI promoter, MFalpha1, 2 micron Marker(s):ampR Construct size (kb): 8.199999809265137 Features: marker(s): ampR promoter: TPI replicon: pMB1, 2 micron |
| Applications： | contains easily purifiable cassette(s) for construction |
| Comments： | Contains EcoRI, PstI, HindIII, BamHI and BglII sites. Restriction digests of the clone give the following sizes (kb): EcoRI--4.2, 1.8, 1.3; HindIII--8.2; BamHI--8.2. A plasmid containing the S. cerevisiae triosephosphate isomerase promoter fused to the signal sequence for mating factor alpha 1 on a BglII/HindIII fragment. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Murray MJ, Kelly JD. Expression of biologically active PDGF analogs in eucaryotic cells. US Patent 4,766,073 dated Aug 23 1988 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |