微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pLUC+ |
|---|---|
| 商品货号： | TS157145 |
| Designations： | pLUC+ |
| Depositors： | J Duschl, J Altschmied |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.5529999732971190 Vector: pLUC+ (plasmid) Marker(s):ampR Construct size (kb): 4.552999973297119 Features: marker(s): ampR replicon: pMB1 reporter group: luciferase (luc+) MCS: HindIII...BamHI MCS: XhoI...EcoRI terminator: SV40 large T-antigen polyadenylation terminator: SV40 late polyadenylation |
| Applications： | promoter-cloning vector reporter construct shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.6; EcoRI--4.6; HindIII--4.6. The vector contains modified luciferase (luc+) gene from the firefly Photinus pyralis in order to increase the yield of recoverable luciferase activity after transfection and to eliminate potential cryptic regulatory elements. The optimized luciferase reporter gene vectors (TS157145 - 87633) provide valuable tools for the analysis of eukaryotic regulatory DNA elements. Promoter-cloning vector for analysis of eukaryotic promoters, using the luciferase reporter gene. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Altschmied J, Duschl J. Set of optimized luciferase reporter gene plasmids compatible with widely used CAT vectors. BioTechniques 23: 436-438, 1997. PubMed: 9298214 |
| Related Products： | component of:ATCC 87654 |
| Shipped： | freeze-dried |
| Shipping Information： | xa0Freeze dried Escherichia coli containing the plasmid. |