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| 产品名称： | pG57/T1 |
|---|---|
| 商品货号： | TS157359 |
| Designations： | pG57/T1 |
| GenBank Number： | M61773 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | A Tzagoloff |
| Applications： | in another host, produces protein mitochondrial protein, required for assembly of F1 ATPase |
| Vector： | Construct size (kb): 16.79998970031738 |
| Insert： | DNA: genomic Insert lengths(kb): 9.0 Gene product: mitochondrial protein, required for assembly of F1 ATPase ATP12 Target Gene: mitochondrial protein, required for assembly of F1 ATPase |
| Insert Size (kb)： | 9.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): SmaI/SalI--10.0, 8.0; EcoRI--6.6, 3.6 (doublet), 2.3, 0.9; PstI--13.5, 2.9. The insert contains the following restriction sites (approximate kb from the end closer to the vector SmaI site nt of the published sequence): XbaI--1.1, 2.8; EcoRI--1.6, 5.3, 6.3 193; SstI--6.1 2; HindIII--7.5 1425. The EcoRI site at nt 193 is within the reading frame. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Bowman S, et al. Characterization of ATP12, a yeast nuclear gene required for the assembly of the mitochondrial F1-ATPase. J. Biol. Chem. 266: 7517-7523, 1991. PubMed: 1826907 |