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| 产品名称： | pM |
|---|---|
| 商品货号： | TS157483 |
| Designations： | pM |
| Depositors： | JJ McCormick |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.1690001487731930 Vector: pM (plasmid) Promoters: Promoter lac Construction: pUC19, pMAMneo, SV40 early splicing region Marker(s):ampR Construct size (kb): 5.169000148773193 Features: insert detection: lacZ marker(s): ampR promoter: lac, MMTV LTR replicon: pMB1 terminator: SV40 enhancer: RSV |
| Applications： | expression vector shuttle vector vector permitting construction of fusion proteins vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): PvuI--3.4, 1.8; EcoRI--2.6, 2.6; BglII--5.2; ClaI--4.1, 1.1. The plasmid contains the following restriction sites (bp from 0): NotI--0; EcoRI--9, 2640; ClaI--425, 1560; DrdI--1398, 3152, 5021; BglII--1605; SphI--2476; NsiI--2478; PvuI--2522, 4310; HindIII--2691; BsaI--4010. Expression vector containing two multiple cloning sites: the first being within the lacZ gene, and the second being flanked by an RSV enhancer-MMTV LTR promoter at one end and splicing and polyadenylation signals at the other. The first site was designed for insertion of a marker selectable in mammalian cells, and the second for the sequence to be expressed. The first site permits detection of inserts by alpha complementation. The order of the major features in this plasmid is: NotI/EcoRI - 5 RSV enhancer - MMTV LTR 3 - ClaI/MCSII/BglII - splicing - polyadenylation - NsiI - 3 lacZ - EcoRI/MCSI/HindIII - 5 lacZ - pMB1 ori - ampR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Wang Q, et al. Mammalian expression vectors with modulatable promoters and two multiple cloning sites. Gene 119: 155-161, 1992. PubMed: 1327962 J J McCormick, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Freeze dried phagemid in E. coli |