微信小陈
微信小章| 产品名称： | pCaW260 |
|---|---|
| 商品货号： | TS157488 |
| Designations： | pCaW260 |
| Species： | Cauliflower mosaic virus |
| Depositors： | JE Schoelz |
| Vector： | Construct size (kb): 12.39400005340576 |
| Insert： | DNA: genomic Insert lengths(kb): 8.0 |
| Insert Size (kb)： | 8.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Shipped: xa0Freeze dried E. coli containing the plasmid |
| Comments： | The clone contains the following restriction sites (using the viral numbering scheme from 1 to the SalI site at 4833): BamHI--1926, 2148, 5108; EcoRI--2414, 2474, 5483, 10009, 10468; SacI--10185; PvuII--4000, 7780, 10681; SalI--4833, 9196. Restriction digests of the clone give the following sizes (kb): SalI--8.0, 4.3; EcoRI--4.3, 4.2, 3.8, 0.4; HindIII--4.1, 3.8, 1.1, 1.0, 0.6 (triplet) 0.3; PstI--8.4, 3.9. Known genes have these approximate position (virus numbering scheme): I: 300-1300; II (aphid helper component): 1300-1800; III: 1800-2200; IV (coat protein): 2200-3600; V (reverse transcriptase): 3600-5600; VI (inclusion body protein): 5800-7300. This strain was isolated from crucifers in the Mendoza province of Argentina. This was constructed by linearizing the full-length CaMV DNA with SalI and inserting it into the SalI site of pBR322. |
| Classification： | Caulimovirus |
| References： | Gardner RC, et al. The complete nucleotide sequence of an infectious clone of cauliflower mosaic virus of M13mp7 shotgun sequencing. Nucleic Acids Res. 9: 2871-2888, 1981. PubMed: 6269062 Gracia O, Shepherd RJ. Cauliflower mosaic virus in the nucleus of Nicotiana. Virology 146: 141-145, 1985. Scholelz JE, Shepherd RJ. Host range control of cauliflower mosaic virus. Virology 162: 30-37, 1988. PubMed: 3341113 James E Schoelz, personal communication |