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| 产品名称： | pRShMR D4S3136, UTR-9757 |
|---|---|
| 商品货号： | TS157502 |
| Designations： | pRShMR D4S3136, UTR-9757 |
| GenBank Number： | M16801 |
| Species： | Homo sapiens, human |
| Depositors： | Salk Institute for Biological Studies, RM Evans, Salk Institute for Biological Studies |
| Applications： | in another host, produces protein mineralocorticoid receptor (aldosterone receptor) |
| Vector： | Construct size (kb): 7.269999980926514 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.369999885559082 Tissue: kidney Gene product: mineralocorticoid receptor (aldosterone receptor) MLR Target Gene: mineralocorticoid receptor (aldosterone receptor) |
| Insert Size (kb)： | 3.370 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): AccI--6.6; AvaI--6.6; EcoRI--2.5, 1.7, 1.4, 1.3. The insert contains the following restriction sites (nt position according to the published sequence): XbaI--258, 1924; SacI--733; BamHI--1273; EcoRI--1770, 3040; AvaI--3425. The 3 HindIII site and the 5 AccI site were lost in cloning. The DNA-binding domain of the protein extends from aa 603 to aa 668 (encoded from nt 2029 to 2226). The ligand-binding domain of the protein extends from aa 734 to aa 984. Contains the AccI/HindIII (nt 188 to nt 3562) fragment from lambdahK10, which includes the complete coding sequence (nt 217 to nt 3171). Expresses functional receptor when transfected into COS-1 cells. The 1.27 kb EcoRI fragment (nt 1770 to nt 3040) has been used to detect mRNA in several rat tissues. The EcoRI fragment of lambdahk10 (nt 1 to nt 3750) was inserted into pGEM-4, excised with HindIII (polylinker to nt 3562), and ligated to the HindIII/HpaI fragment of pRSVcat. The AccI fragment (about 200 bp) at the 5 end was removed to improve translation, and the PvuII/HindIII fragment from pSV2cat containing the SV40 ori was linkered with NdeI and inserted at the NdeI site. |
| References： | Evans RM. The steroid and thyroid hormone receptor superfamily. Science 240: 889-895, 1988. PubMed: 3283939 Evans RM, et al. Retinoic acid receptor method. US Patent 4,981,784 dated Jan 1 1991 Arriza JL, et al. Cloning of human mineralocorticoid receptor complementary DNA: structural and functional kinship with the glucocorticoid receptor. Science 237: 268-275, 1987. PubMed: 3037703 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |