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| 产品名称： | pHred24 pHRed24 |
|---|---|
| 商品货号： | TS157542 |
| Designations： | pHred24 pHRed24 |
| GenBank Number： | M15959 |
| Species： | Homo sapiens, human |
| Depositors： | KL Luskey |
| Vector： | Construct size (kb): 6.599999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 2.299999952316284 Gene product: 3-hydroxy-3-methylglutaryl-Coenzyme A reductase HMGCR Alleles: A1, A2 |
| Insert Size (kb)： | 2.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Insert contains internal PvuII (1), PstI (2), BglII (2) and BamHI (1) sites. Restriction digests of the clone give the following sizes (kb): SphI--4.4, 2.2; EcoRI--6.6; HindIII--6.6; BamHI--6.0, 1.0; PstI--3.6, 1.65, 1.2. Clone of the 5-end of HMG-CoA including the 5 initiation sites and exon 1. Enzyme(s) not detecting polymorphism: ApaI, ApaLI, Asp718, AvaII, BamHI, BglI, EcoRI, NcoI, NheI, PstI, PvuII, SacI, SpeI, StuI (8 unrelated individuals). |
| References： | Luskey KL. Conservation of promoter sequence but not complex intron splicing pattern in human and hamster genes for 3-hydroxy-3-methylglutaryl coenzyme A reductase. Mol. Cell. Biol. 7: 1881-1893, 1987. PubMed: 3037337 Leitersdorf E, Luskey KL. HgiAl polymorphism near the HMGCR promoter. Nucleic Acids Res. 18: 5584, 1990. PubMed: 1977131 |