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| 产品名称： | pCMV5-hpbr pCMV-5PBR, pCMV5-PBR |
|---|---|
| 商品货号： | TS157543 |
| Designations： | pCMV5-hpbr pCMV-5PBR, pCMV5-PBR |
| GenBank Number： | M36035 |
| Species： | Homo sapiens, human |
| Applications： | in another host, produces protein benzodiazapine receptor (peripheral) |
| Vector： | Construct size (kb): 5.550000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.8510000109672546 Tissue: liver Gene product: benzodiazapine receptor (peripheral) BZRP Target Gene: benzodiazapine receptor (peripheral) |
| Insert Size (kb)： | 0.851 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.7, 0.85; BglI--1.6, 1.25, 0.95, 0.68, 0.45, 0.35, 0.3; KpnI--5.1, 0.5; PstI--5.0, 0.45, 0.1; SacI--5.0, 0.9. The insert contains the following restriction sites (bp from the 5 end): BglI--212, 519; KpnI--470; PstI--83, 202, 623; SacI--64; SmaI--761. BZRP has been localized to 22q13.31 on the human chromosome. a human genomic EcoRI fragment of approximately 7 kb, and a human genomic HindIII fragment of 6.5 kb. The hamster genomic EcoRI fragment is larger. Contains the complete coding sequence which is identical to that reported in GenBank accession M36035. This insert also has a longer 5 untranslated region. Detects RNA of approximately 1 kb in Northern blots, The insert was cloned into a shuttle vector that regulates expression of inserts by the cytomegalovirus (CMV) major early immediate promoter and contains the human growth hormone (GH) terminator. |
| References： | Chang YJ, et al. The human "pripheral-type" benzodiazepine receptor: regional mapping of the gene and characterization of the receptor expressed from cDNA. DNA Cell Biol. 11: 471-480, 1992. PubMed: 1326278 Riond J, et al. Molecular cloning and chromosomal localization of a human peripheral-type benzodiazepine receptor. Eur. J. Biochem. 195: 305-311, 1991. PubMed: 1847678 Jerome F Strauss, personal communication |