微信小陈
微信小章| 产品名称： | 121-19B10 |
|---|---|
| 商品货号： | TS157585 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue： | spleen |
| Cell Type： | hybridoma:lymphoblast B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | female |
| Strain： | 129GIX+ |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | female |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5 Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG2a and 2b kappa (Only the IgG2a immunoglobulin is reactive with the antigen.) |
| Name of Depositor： | National Cancer Institute |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Animals were immunized with the synthetic peptide IGRGNFGEVFSG(C) corresponding to residues 567 through 578 of the FES sequence (formerly residues 529 through 540 of the HC/v-fes sequence). Only the IgG2a immunoglobulin is reactive with the antigen. In immunoblot assays, the antibody detects an 85 kd gag-fes fusion protein encoded by the Gardner-Arnstein strain of feline sarcoma in viral transformed mink lung cells. In an ELISA test, the antibody does not cross-react with the homologous region of src, yes, erb B, mil, raf, fms, fqr, abl, mos, CDC28 or the regulatory or catalytic subunits of bovine cyclic AMP dependent protein kinase. The IgG2a and IgG2b reactivities of the antibody with the fes peptide (567 to 578) were confirmed by ELISA. IgG2a was strongly positive while IgG2b was negative. Only the IgG2a immunoglobulin is reactive with the antigen. |