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| 产品名称： | pAS64-H4 plasmid in E. coli full-length clone of tomato apical stunt viroid |
|---|---|
| 商品货号： | TS157615 |
| Designations： | pAS64-H4 plasmid in E. coli full-length clone of tomato apical stunt viroid |
| Depositors： | RA Owens |
| Vector： | Total size of construct (kb): 3.36 Marker: ampR Excise insert with: HindIII Vector: pSP64 Vector type: plasmid Vector size (kb): 3.0 Vector ends: HindIII Markers: ampR |
| Insert： | Insert size (kb): 0.36 Gene: Full-length Tomato Apical Stunt Viroid, TASV DNA: cDNA Strain: Ivory Coast Insert end: HindIII |
| Insert Size (kb)： | 0.36 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | In E. coli containing the plasmid |
| Comments： | The insert contains internal PstI, BamHI and SmaI sites. PstI digestion is diagnostic for the insert orientation. Restriction digests of the clone give the following sizes (kb): HindIII--3.1, 0.36; PstI--3.2, 0.27; EcoRI--3.4. Full-length (360 bp) cDNA fragment of TASV with HindIII ends was cloned into HindIII site of pSP64; noninfectious. |
| References： | Kiefer MC, et al. Structural similarities between viroids and transposable genetic elements. Proc. Natl. Acad. Sci. USA 80: 6234-6238, 1983. PubMed: 6312450 Robert A Owens, personal communication |