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| 产品名称： | c8025 |
|---|---|
| 商品货号： | TS157632 |
| Designations： | c8025 |
| GenBank Number： | U00061 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | L Riles, MV Olson |
| Vector： | Construct size (kb): 36.79999923706055 |
| Insert： | DNA: genomic Insert lengths(kb): 30.34900093078613 Gene product: peptidyl-prolyl cis-trans isomerase (cyclophilin, FK506 binding protein) CPR2 |
| Insert Size (kb)： | 30.349 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Reported to contain genomic EcoRI/HindIII fragments of the following sizes (kb): 5.62, 3.24, 3.15, 2.64, 1.77, 1.11, 1.03, 0.94, 0.76, 0.55, 0.5. Also contains fragments of 14.94 (CUP1?) and 8.4 (vector-associated). IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Corresponds to nt 209622-240071 of the sequence of chromosome VIII. RPP1 is an essential gene encoding a 32.2 kD ortholog of the human scleroderma autoimmune antigen Rpp30. The gene is contained in a 1.4 kb BamHI/XbaI fragment. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Stolc V, Altman S. Rpp1, an essential protein subunit of nuclear RNase P required for processing of precursor tRNA and 35S precursor rRNA in Saccharomyces cerevisiae. Genes Dev. 11: 2414-2425, 1997. PubMed: 9308968 Johnston M, et al. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. Science 265: 2077-2082, 1994. PubMed: 8091229 Linda Riles, personal communication |