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| 产品名称： | pACT2 pACTII |
|---|---|
| 商品货号： | TS157720 |
| Designations： | pACT2 pACTII |
| Depositors： | SJ Elledge |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli |
| Vector Information： | Size (kb): 7.5500001907348630 DESCRIPTION OF VECTOR: Intact vector size: 7.550 Type of vector: plasmid Cloning sites: NcoI SfiI SmaI BamHI EcoRI XhoI BglII Polylinker sites: NdeI NcoI SfiI SmaI BamHI EcoRI XhoI BglII Construction: pSE1107 (pACT1) Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli Features (with orientation and position when available): promoter: ADC1 (ADH), -> other: GAL4 activation domain, -> epitope tag: hemagglutinin (HA), -> MCS: BglII/NdeI...BglII, -> terminator: ADC1 marker(s): LEU2, replicon: 2 micron marker(s): ampR replicon: pMB1 Vector: pACT2 (plasmid) Promoters: Promoter ADC1 (ADH) Construction: pSE1107 (pACT1) Marker(s):LEU2,ampR Construct size (kb): 7.550000190734863 Features: marker(s): LEU2 marker(s): ampR other: GAL4 activation domain promoter: ADC1 (ADH) replicon: 2 micron replicon: pMB1 MCS: BglII/NdeI...BglII terminator: ADC1 epitope tag: hemagglutinin (HA) |
| Applications： | YE-type (episomal) shuttle vector expression vector vector component of two-hybrid screening system vector for constructing cDNA libraries |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--7.6; BamHI--7.6; BglII--7.5. Shuttle expression vector used to create fusion proteins consisting of the nuclear localization sequence from SV40 T antigen, the GAL4 activation domain (aa 768-881) and a HA (hemagglutinin) epitope tag in frame with the activation domain. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Stephen J Elledge, personal communication |
| Related Products： | component of:ATCC 87014 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |