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| 产品名称： | pG53/T1 |
|---|---|
| 商品货号： | TS157817 |
| Designations： | pG53/T1 |
| GenBank Number： | X55731 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | A Tzagoloff |
| Applications： | in another host, produces protein mitochondrial membrane protein, required for assembly of cytochrome oxidase |
| Vector： | Construct size (kb): 20.29998970031738 |
| Insert： | DNA: genomic Insert lengths(kb): 9.5 Gene product: mitochondrial membrane protein, required for assembly of cytochrome oxidase COX11 Target Gene: mitochondrial membrane protein, required for assembly of cytochrome oxidase |
| Insert Size (kb)： | 9.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--6.6, 4.4 (doublet), 3.0, 2.0; EcoRI--5.2, 4.6 (doublet), 2.3 (doublet), 1.2; PstI--7.6, 5.8, 4.2, 1.7, 1.0; HindIII--11.0, 4.4, 3.6, 1.2. The insert contains the following restriction sites (approximate kb from the end distal to the vector HindIII site nt of the published sequence): HindIII--0.4, 4.8, 8.6; EcoRI--0.4 42, 1.9 1190, 7.4; BglII--1.5 797, 3.6, 7.8. The gene product is homologous to ORF3 of Paracoccus denitrificans. The sequenced portion (the 1.87 kb BamHI/XbaI fragment) also contains an open reading frame transcribed in the opposite orientation. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Tzagoloff A, et al. Cytochrome oxidase assembly in yeast requires the product of COX11, a homolog of the P. denitrificans protein encoded by ORF3. EMBO J. 9: 2759-2764, 1990. PubMed: 2167832 |