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微信小章| 产品名称： | pMCMP1 |
|---|---|
| 商品货号： | TS157889 |
| Designations： | pMCMP1 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Vector： | Construct size (kb): 4.800000190734863 |
| Insert： | DNA: genomic Insert lengths(kb): 2.299999952316284 Gene product: phosphorylase, glycogen; muscle (McArdle syndrome, glycogen storage disease type V) PYGM Alleles: A1, A2, A3 |
| Insert Size (kb)： | 2.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): AccI--4.8; BamHI--4.8; EcoRI--3.0, 1.75; HindIII--4.8; PvuII--2.4, 1.0, 0.85, 0.48. Evidence of linkage (theta = 0.00, LOD score = 7.55) was found between the skeletal muscle glycogen phosphorylase (PYGM) gene on 11q and multiple endocrine neoplasia type 1 (MEN1). Polymorphism also detected with TaqI. Hybridization solution contained about 100 ug/ml total human DNA and 50% formamide. Subcloned from cosmid cMCMP1 isolated by using the HM11 3 myophosphorylase cDNA. Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, HindIII, RsaI. |
| References： | Larsson C, et al. Multiple endocrine neoplasia type 1 gene maps to chromosome 11 and is lost in insulinoma. Nature 332: 85-87, 1988. PubMed: 2894610 Carlson M, et al. Isolation and mapping of a polymorphic DNA sequence for human muscle glycogen phosphorylase (pMCMP1) on chromosome 11 PYGM. Nucleic Acids Res. 16: 10403, 1988. PubMed: 2461546 Nakamura Y, et al. Localization of the genetic defect in multiple endocrine neoplasia type I within a small region of chromosome 11. Am. J. Hum. Genet. 44: 751-755, 1989. PubMed: 2565085 Peter J OConnell, personal communication |