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微信小章| 产品名称： | Ectromelia virus |
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| 商品货号： | TS157891 |
| Classification： | Poxviridae, Orthopoxvirus |
| Agent： | Ectromelia virus |
| Strain： | Moscow |
| Applications： | 1982. Mousepox, (in) The Mouse in Biomedical Research: Vol. Requests for the agent must carry a signed statement assuming all risk and responsibility for handling and use. |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Comments： | Inadvertent introduction of Ectromelia virus into mouse colonies has caused major outbreaks in US animal colonies. The depositor recommends that animal care and biosafety management at your institution be consulted prior to initiating work with this agent. Requests for the agent must carry a signed statement assuming all risk and responsibility for handling and use. For additional information see: Fenner. 1982. Mousepox, (in) The Mouse in Biomedical Research: Vol. II. Diseases, (ed) Foster et al., Academic Press. |
| Effect on Host： | CPE, refractile rounding; death in mice |
| Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
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| Recommended Host： |
BS-C-1 (ATCC® CCL-26™)
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| Growth Conditions： | Incubation: 1 to 7 days at 37°C, a 5% CO2 in air atmosphere is recommended Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent). |
| Name of Depositor： | RM Buller |
| Special Collection： | ATCC |
| Source： | Spleen homogenate from BALB/c mouse 6 days post-infection |
| References： | Chen W, et al. Restricted replication of ectromelia virus in cell culture correlates with mutations in virus-encoded host range gene. Virology 187: 433-442, 1992. PubMed: 1546448 |
| Cross References： | Nucleotide (GenBank) : AF012825 Ectromelia virus envelope glycoprotein C1R (C1R), C2R (C2R), C3R (C3R), IFN-gamma binding protein C4R (C4R), C5R (C5R), protein kinase C6R (C6R), serpin C7R (C7R), C8R (C8R), IL-1 beta binding protein C9R (C9R), C10L (C10L), C11R (C11R), IFN-... |