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| 产品名称： | PSN-1 |
|---|---|
| 商品货号： | TS157896 |
| Organism： | Homo sapiens, human |
| Tissue： | pancreas |
| Product Format： | frozen |
| Morphology： | epithelial-like |
| Culture Properties： | adherent |
| Biosafety Level： | 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma |
| Storage Conditions： | liquid nitrogen vapor phase |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze Medium: Complete growth medium supplemented with an additional 10% heat inactivated fetal bovine serum and 10% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 35°C to 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |