微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pRD112 plasmid in E. coli |
|---|---|
| 商品货号： | TS157914 |
| Designations： | pRD112 plasmid in E. coli |
| Species： | Aspergillus niger var. macrosporus Koaze, anamorph |
| Depositors： | OmniGene, Inc., RR Yocum, OmniGene, Inc. |
| Applications： | produces protein glucoamylase |
| Vector： | Construct size (kb): 8.30 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGM30 Intact vector size: 8.800 Type of vector: plasmid Vector end: XhoI Modification: BssHII adaptor Vector end: XbaI Cloning sites: XbaI XhoI Polylinker sites: Construction: pRY271, pGM11 Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): URA3 promoter: TPI leader peptide: DEX4 coding sequence: DEX4 transcription terminator: TPI replicon: pMB1 marker(s): ampR replicon: 2 micron Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Aspergillus niger var. macrosporus Gene name: glucoamylase Contains complete coding sequence?: Y Type of DNA: cDNA Insert 5 end: BssHII Insert 3 end: XbaI Insert size (kb): 2.2 Cross references: Insert lengths(kb): 2.20 Gene product: glucoamylase Target Gene: glucoamylase |
| Insert Size (kb)： | 2.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI/XhoI--6.6, 2.1; SmaI--8.6; XbaI--8.6. When transformed into yeast strain RD61-1D, pRD112 yields approximately 100 units of glucoamylase activity. |
| References： | Maine GT, et al. Expression and secretion vector for yeast containing a glucoamylase signal sequence. US Patent 5,024,941 dated Jun 18 1991 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |