微信小陈
微信小章
产品简介
购买须知
| 产品名称： | ptkLUC+ |
|---|---|
| 商品货号： | TS158026 |
| Designations： | ptkLUC+ |
| Depositors： | J Duschl, J Altschmied |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 4.7109999656677250 DESCRIPTION OF VECTOR: Intact vector size: 4.711 Type of vector: plasmid Cloning sites: HindIII SalI BamHI ClaI SmaI KpnI SacI Polylinker sites: HindIII SalI BamHI tk-promoter luc+ SV40 poly (A) ClaI SmaI KpnI SacI Other unique sites: NarI BclI FseI ClaI SmaI KpnI SacI PvuII BsaI BglI FspI PvuI XmnI SspI Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): MCS: HindIII...BamHI, ->, 1-36 promoter: TK, 37-200 reporter group: luciferase (luc+), ->, 238-1890 terminator: SV40 late polyadenylation, 1922-2143 MCS: ClaI...SacI, 2146-2168 replicon: pMB1, 2587-2587 marker(s): ampR, terminator: SV40 large T-antigen polyadenylation, 4337-4708 Vector: ptkLUC+ (plasmid) Promoters: Promoter TK Marker(s):ampR Construct size (kb): 4.710999965667725 Features: marker(s): ampR promoter: TK replicon: pMB1 reporter group: luciferase (luc+) MCS: ClaI...SacI MCS: HindIII...BamHI terminator: SV40 large T-antigen polyadenylation terminator: SV40 late polyadenylation |
| Applications： | reporter construct
xa0shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.7; HindIII--4.7. The vector contains modified luciferase (luc+) gene from the firefly Photinus pyralis in order to increase the yield of recoverable luciferase activity after transfection and to eliminate potential cryptic regulatory elements. The promoters driving luc+ transcription in these two vectors represent different 5 deletions of the tk promoter ending at -32 and -105, respectively. The optimized luciferase reporter gene vectors (ATCC 87630 - 87633) provide valuable tools for the analysis of eukaryotic regulatory DNA elements. The vectors pTATALUC+ (ATCC 87631) and ptkLUC+ (TS158026) were designed for the characterization of isolated cis regulatory elements. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Altschmied J, Duschl J. Set of optimized luciferase reporter gene plasmids compatible with widely used CAT vectors. BioTechniques 23: 436-438, 1997. PubMed: 9298214 |
| Related Products： | component of:ATCC 87654 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |