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| 产品名称： | 1G12 |
|---|---|
| 商品货号： | TS158038 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte; somatic cell hybri |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | CB.17 |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | Animals were immunized with T cells from 3A9 T cell receptor (TCR) transgenic mice. Spleen cells were fused with P3X63Ag8 myeloma cells.The antibody recognizes the 3A9 T cell receptor (TCR). |
| Comments： | Animals were immunized with T cells from 3A9 T cell receptor (TCR) transgenic mice. Spleen cells were fused with P3X63Ag8 myeloma cells. The antibody recognizes the 3A9 T cell receptor (TCR). |
| Complete Growth Medium： | Dulbeccos Modified Eagles Medium with 4 mM L-glutamine that is modified by ATCC to contain 4.5 g/L glucose and 1.5 g/L sodium bicarbonates supplemented with 1.5 mM L-glutamine, 0.05 mM 2-mercaptoethanol, 10 mM HEPES, 116mg/L L-arginine HCl, 41 mg/L L-asparagine.H2O and 10% heat-inactivated fetal bovine serum
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| Subculturing： | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 8 X 10(4) viable cells/ml. Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with an additional 10% heat-inactivated fetal bovine serum and 7.5% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype： | IgG1 kappa |
| Population Doubling Time： | 10 hours |
| Name of Depositor： | ER Unanue |
| Year of Origin： | 1996 |
| References： | Van Parijs L, et al. The Fas/Fas ligand pathway and Bcl-2 regulate T cell responses to model self and foreign antigens. Immunity 8: 265-274, 1998. PubMed: 9492007 Peterson DA, et al. Quantitative analysis of the T cell repertoire that escapes negative selection. Immunity 11: 453-462, 1999. PubMed: 10549627 |