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| 产品名称： | pGB2862 |
|---|---|
| 商品货号： | TS158040 |
| Designations： | pGB2862 |
| GenBank Number： | M17697 |
| Species： | Homo sapiens, human |
| Applications： | This clone encodes conserved epitopes because it was isolated from an expression library by anti-bovine glutamate dehydrogenase antibodies. |
| Vector： | Construct size (kb): 5.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.799999952316284 Tissue: brain stem Gene product: glutamate dehydrogenase 1 GLUD |
| Insert Size (kb)： | 1.800 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | The insert contains the following sites separated by (bp)(approx): EcoRI- 340- BglII- 50- BglII- 210- EcoRV- 305- PstI- 920- EcoRI. Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.1; BamHI--5.0; HincII--3.2, 1.4 + smaller; BglII--5.2; XbaI--5.2. The insert contains a 774 bp open reading frame which codes for amino acids 244-501 of human liver GDH, and about 1 kb of untranslated sequence. The cDNA sequence of human brainstem glutamate dehydrogenase is 95% homologous to the human liver sequence and 97% homologous to the bovine liver sequence. This probe detects a single 3.7 kb band in human brain and glioma mRNA. In rat it detects a 3.5 kb band in liver, brain and C6 glioma cells, a 2.8 kb band in kidney, and a reduced 2.8 kb band in brain total mRNA. This clone encodes conserved epitopes because it was isolated from an expression library by anti-bovine glutamate dehydrogenase antibodies. |
| References： | Banner C, et al. Isolation of a human brain cDNA for glutamate dehydrogenase. J. Neurochem. 49: 246-252, 1987. PubMed: 3585334 |