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| 产品名称： | YEp432 pRB693 |
|---|---|
| 商品货号： | TS158176 |
| Designations： | YEp432 pRB693 |
| Depositors： | D Botstein |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.4000000953674320 Vector: YEp432 (plasmid) Construction: YEp420, pBR315 Marker(s):TRP1,ampR Construct size (kb): 6.400000095367432 Features: marker(s): ampR, TRP1 replicon: pMB1, 2 micron |
| Applications： | YE-type (episomal) shuttle vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/BamHI--5.6, 0.9; EcoRI/SalI--5.4, 1.1; XbaI--3.3, 3.0. YE-type shuttle vector. The order of the major features in this plasmid is: EcoRI site - TRP1 - BamHI site - SalI site - ori(2 micron) - ampR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Ma H, et al. Plasmid construction by homologous recombination in yeast. Gene 58: 201-216, 1987. PubMed: 2828185 |
| Shipped： | freeze-dried |