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微信小章| 产品名称： | P-1 P1 |
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| 商品货号： | TS158240 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue： | spleen |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | BALB/c |
| Applications： | The antibody reacts with the small subunit of bovine mu-caplain and m-calpain. It cross-reacts with the small subunit of human and canine mu-caplain and m-calpain. The antibody can be used for immunochemical and Western blot applications. It is a useful tool for detecting and localizing the small subunit of calpain. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with purified bovine myocardial calpain 2 Spleen cells were fused with FOX-NY myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against the small subunit of calpain |
| Cellular Products： | immunoglobulin; monoclonal antibody; against the small subunit of calpain |
| Comments： | Animals were immunized with purified bovine myocardial calpain 2 Spleen cells were fused with FOX-NY myeloma cells. The antibody reacts with the small subunit of bovine mu-caplain and m-calpain. It cross-reacts with the small subunit of human and canine mu-caplain and m-calpain. The antibody can be used for immunochemical and Western blot applications. It is a useful tool for detecting and localizing the small subunit of calpain. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 x 104 viable cells/mL. Maintain cell concentration between 3 x 104 and 3 x 105 viable cells/mL. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG1 kappa , IgG1; kappa light chain |
| Population Doubling Time： | 24 hours |
| Name of Depositor： | R Lane, RL Mellgren |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| Year of Origin： | 1986 |
| References： | Mellgren RL, Lane RD. Myocardial calpain 2 is inhibited by monoclonal antibodies specific for the small, noncatalytic subunit. Biochim. Biophys. Acta 954: 154-160, 1988. PubMed: 2835105 Zhang W, Mellgren RL. Calpain Subunits Remain Associated during Catalysis. Biochem. Biophys. Res. Commun. 227: 890-896, 1996. PubMed: 8954990 Zhang W, et al. The major calpain isozymes are long-lived proteins. Design of an antisense strategy for calpain depletion in cultured cells. J. Biol. Chem. 271: 18825-18830, 1996. PubMed: 8702541 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |