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| 产品名称： | 07-03 pPM101 |
|---|---|
| 商品货号： | TS158315 |
| Designations： | 07-03 pPM101 |
| Species： | Homo sapiens, human |
| Applications： | X linked segregation observed in 3 families with 31 progeny. A 2.3 kb EcoRI fragment has been used to detect a BanII RFLP. Enzyme(s) not detecting polymorphism: ApaI, BamHI, BglII, BstEII, BstNI, CfoI, EcoRI, EcoRV, HaeIII, HindII, HindIII, HinfI, HpaII, KpnI, MspI, PstI, PvuII, RsaI, SacI, ScaI, TaqI, XbaI, XhoI, XmnI (tested against 10-12 unrelated Caucasians). |
| Vector： | Construct size (kb): 50.0 |
| Insert： | DNA: genomic Insert lengths(kb): 18.0 Gene product: DNA Segment, single copy DXS79 Alleles: A1, A2 |
| Insert Size (kb)： | 18.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--23, 9.4 (arms); 18 (insert); EcoRI--20 (arm), 13 (arm/insert), 3.2, 3.0, 2.4, 1.8/1.75 doublet. X linked segregation observed in 3 families with 31 progeny. Informative in CEPH pedigrees 21, 23, 1331, 1345, and IMR pedigree 884. A 2.3 kb EcoRI fragment has been used to detect a BanII RFLP. The 2.4 (2.3) kb EcoRI fragment is unique and detects the RFLP; other fragments contain repeats. Enzyme(s) not detecting polymorphism: ApaI, BamHI, BglII, BstEII, BstNI, CfoI, EcoRI, EcoRV, HaeIII, HindII, HindIII, HinfI, HpaII, KpnI, MspI, PstI, PvuII, RsaI, SacI, ScaI, TaqI, XbaI, XhoI, XmnI (tested against 10-12 unrelated Caucasians). |
| References： | Murphy PD, et al. An anonymous single copy X-chromosome clone, DXS79, from Xq26-Xq28, identifies a moderately frequent RFLP. Nucleic Acids Res. 13: 3015, 1985. PubMed: 4000970 Murphy PD, Ruddle FH. Isolation and regional mapping of random X sequences from distal human X chromosome. Somatic Cell Mol. Genet. 11: 433-444, 1985. PubMed: 2994237 Patricia D Murphy, personal communication |