微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pMAMPF-3 |
|---|---|
| 商品货号： | TS158343 |
| Designations： | pMAMPF-3 |
| Depositors： | J Collins |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.3540000915527340 Vector: pMAMPF-3 (phagemid) Promoters: Promoter lambda PL Construction: pMAC5-14; pINOmpA3-NaeI Marker(s):ampR Construct size (kb): 4.354000091552734 Features: marker(s): ampR promoter: lambda PL replicon: pMB1, fd |
| Applications： | expression vector vector containing primer sites useful for sequencing vector permitting construction of fusion proteins vector permitting production of single-stranded DNA vector with sequences for protein export |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI--4.3; PstI--2.3, 2.0; XbaI--4.3; EcoRI--4.3; HindIII--4.3. Expression vector permitting production of a fusion protein and containing signal sequences for protein export. Also permits production of ssDNA and contains priming sites useful for sequencing. Encodes the ompA leader. Contains a chloramphenicol acetyltransferase gene with an amber mutation useful for "gapped-duplex" mutagenesis experiments. Expression can be induced by a temperature shift in hosts providing a temperature-sensitive lambda repressor, or by batch transduction in a growing culture to repressor-negative hosts. The order of the major features in this phagemid is: lambdaPL promoter - ompA leader - NaeI site - MCS - priming site - fd ori - pMB1 ori - bla - cat (am). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Szardenings M, Collins J. A phasmid optimised for protein design projects: pMAMPF. Gene 94: 1-7, 1990. PubMed: 2146188 |
| Shipped： | freeze-dried |