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| 产品名称： | pGBM4 |
|---|---|
| 商品货号： | TS158375 |
| Designations： | pGBM4 |
| Depositors： | J Geiselmann |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.0619997978210450 Vector: pGBM4 (plasmid) Promoters: Promoter E. coli Construction: pSC101 Marker(s):spcR,strR Construct size (kb): 4.061999797821045 Features: marker(s): strR/spcR promoter: E. coli replicon: pSC101 MCS: XhoI...EcoRI |
| Applications： | The vector can be used whenever it is necessary to maintain two plasmids within the same cell. |
| Comments： | Restriction digests of the clone give the following sizes (kb): XhoI--4.1; BglI--3.0, 1.1; AvaII--3.4, 0.7. Appropriate oligonucleotides can be inserted to change the sequences of the -10 and -35 reions of the promoter and thus set the promoter strength to any desired level. The vector can be used whenever it is necessary to maintain two plasmids within the same cell. ATCC 87499 and 87500 differ in -10 region promoter sequences: 87499--TACCTT; 87500--TATCAT. The sequence of -35 region promoter is TTGCAT. |
| Media： | ATCC® Medium 1639: LB medium (ATCC medium 1065) with 50 mcg/ml spectinomycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Manen D, et al. A sensitive reporter gene system using bacterial luciferase based on a series of plasmid cloning vectors compatible with derivatives of pBR322. Gene 186: 197-200, 1997. PubMed: 9074496 |
| Shipped： | frozen |