微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pAB366-2 |
|---|---|
| 商品货号： | TS158392 |
| Designations： | pAB366-2 |
| Depositors： | BM Tyler |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.4000000953674320 Vector: pAB366-2 (plasmid) Construction: pAB366 (ATCC 77134) Marker(s):ampR bleR Construct size (kb): 4.400000095367432 Features: marker(s): ampR bleR replicon: pMB1 |
| Applications： | shuttle vector vector for other uses |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--2.9, 1.5; BamHI/XhoI--3.6, 0.8; BglI--3.0, 1.4. Expression of ble (bleomycin resistance) is regulated by the promoter and terminator regions of the am gene (amination, glutamate dehydrogenase) from N. crassa. Shuttle vector conferring bleomycin and phleomycin resistance. Differs from pAB366 (ATCC 77134) by the insertion of a NsiI cloning site in the 5 untranslated region of ble to permit transcriptional fusion. The order of the major features in this plasmid is: pTZ19R - EcoRI - BamHI - am promoter - NsiI - ble - XhoI - am 3 region - HindIII - pTZ19R. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Austin B, et al. Optimized vectors and selection for transformation of Neurospora crassa and Aspergillus nidulans to bleomycin and phleomycin resistance. Gene 93: 157-162, 1990. PubMed: 1699844 |
| Shipped： | freeze-dried |