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| 产品名称： | human Id-1b phId-1b |
|---|---|
| 商品货号： | TS158447 |
| Designations： | human Id-1b phId-1b |
| GenBank Number： | S78825 |
| Species： | Homo sapiens, human |
| Applications： | contains sequence inhibitor of DNA binding 1, dominant negative helix-loop-helix protein Inhibitor of DNA binding 1, dominant negative, Id1 |
| Vector： | Construct size (kb): 4.099999904632568 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI NotI XmaIII SacII BstXI SacI Polylinker sites: SEE COMMENTS Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, T3, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: ID1 Gene name: inhibitor of DNA binding 1, dominant negative helix-loop-helix protein Contains complete coding sequence?: Y Chromosome: 20; Localization: 20 q11 Tissue: U251 glioblastoma cell line Type of DNA: cDNA Insert end: EcoRI Insert end: EcoRI Insert size (kb): 1.145 Cross references: DNA Seq. Acc.: S78825 Insert lengths(kb): 1.144999980926514 Tissue: U251 glioblastoma cell line Gene product: inhibitor of DNA binding 1, dominant negative helix-loop-helix protein( Inhibitor of DNA binding 1, dominant negative, Id1) ID1 Target Gene: inhibitor of DNA binding 1, dominant negative helix-loop-helix protein |
| Insert Size (kb)： | 1.145 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.2; BglI--2.2, 1.3, 0.7; KpnI--3.5, 0.65; PvuI--2.1, 1.1, 1.0, 0.1; PvuII--2.5, 1.4, 0.3. Insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.42; KpnI--0.54; PvuI--0.70, 0.83; PvuII--0.05; SmaI--0.36; SphI--0.70. There is an erroneous single extra nucleotide "t" at position 1091 in GenBank record S78225. The clones human Id-1a (ATCC 99522/3) and human Id-1b (ATCC 99524/5) represent alternatively spliced products of the same gene. |
| References： | Zhu W, et al. Id gene expression during development and molecular cloning of the human Id-1 gene. Brain Res. Mol. Brain Res. 30: 312-326, 1995. PubMed: 7637581 Mark A Israel, personal communication |