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微信小章| 产品名称： | BJ-5ta |
|---|---|
| 商品货号： | TS158451 |
| Organism： | Homo sapiens, human |
| Tissue： | Foreskin |
| Cell Type： | Fibroblast immortalized with hTERT |
| Product Format： | frozen |
| Morphology： | Fibroblast-like |
| Culture Properties： | Adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Normal |
| Age： | Neonatal |
| Gender： | Male |
| Storage Conditions： | Liquid nitrogen vapor phase |
| Karyotype： | This is a diploid human cell line of male origin with a modal chromosome number of 46 that occurred in 90% of the cells counted. The sex chromosomes, X and Y are both karyotypically normal. |
| Images： |  |
| Derivation： | The hTERT-immortalized foreskin fibroblast cell line, BJ-5ta, was derived by transfecting the BJ foreskin fibroblast cell line with the pGRN145 hTERT-expressing plasmid (ATCC MBA-141) at population doubling 58. Cells were cultured in medium containing hygromycin B until stable clones were selected Pubmed: 9454332.xa0 |
| Antigen Expression： | Antigen expression: Positive for fibroblast surface protein; Homo sapiens, expressed (fibroblast surface protein (FSP) was assayed by flow cytometry.). Negative for the pan-cytokeratin epithelial marker; Homo sapiens (cytokeratins were assayed by immunocytochemistry using a pan-cytokeratin antibody). |
| Complete Growth Medium： | A 4:1 mixture of Dulbeccos medium and Medium 199 with supplements as follows : 4 parts of Dulbeccos Modified Eagles Medium containing 4 mM L-glutamine, 4.5 g/L glucose and 1.5 g/L sodium bicarbonate 1 part of Medium 199 Supplemented with: 0.01 mg/ml hygromycin B 10% fetal bovine serum |
| Subculturing： | Volumes are given for a 75 cm2 flask. Recommended use of Corning® T-75 flasks (catalog #430641). Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:3 twice weekly
Medium Renewal: every 2 to 3 days
Note: Subculture when cell concentration reaches between 8 X 103 and 1 X 104xa0cells/cm2.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Freeze medium: culture medium, 30%; fetal bovine serum, 60%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 8,9 D16S539: 9,13 D5S818: 12 D7S820: 11,12 THO1: 7,8 TPOX: 10,11 vWA: 16,18 |
| Population Doubling Level (PDL)： | As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation. |
| Name of Depositor： | Geron Corporation |
| Deposited As： | Homo sapiens |
| References： | Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332 Jiang XR, et al. Telomerase expression in human somatic cells does not induce changes associated with a transformed phenotype. Nat. Genet. 21: 111-114, 1999. PubMed: 9916802 Aiastui A, et al. Salmonella enterica serovar typhimurium invades fibroblasts by multiple routes differing from the entry into epithelial cells. Infect Immun 78(6):2700-13, 2010. PubMed: 20368348 Janeckova L, et al. HIC1xa0Tumorxa0Suppressorxa0Lossxa0Potentiatesxa0TLR2/NF-κBxa0Signalingxa0andxa0Promotesxa0Tissuexa0Damage-Associatedxa0Tumorigenesis. Mol Cancer Resxa013(7):1139-48, 2015. PubMed: 25934696 Amadori S, et al. Effect of sterilization and crosslinking on gelatin films. J Mater Sci Mater Med 26(2):69, 2015. PubMed: 25631265 La Gatta A, et al. Hyaluronan dermal fillers via crosslinking with 1,4-butandiol diglycidyl ether: Exploitation of heterogeneous reaction conditions. J Biomed Mater Res B Appl Biomater 104(1):9-18, 2016. PubMed: 25611588 |