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| 产品名称： | pUC13 |
|---|---|
| 商品货号： | TS158491 |
| Designations： | pUC13 |
| Depositors： | J Messing |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.7000000476837160 Vector: pUC13 (plasmid) Promoters: Promoter lac Construction: pBR322, Escherichia coli lacZalpha, M13mp11 Marker(s):ampR Construct size (kb): 2.700000047683716 Features: insert detection: lacZ marker(s): ampR promoter: lac replicon: pMB1 terminator: none enhancer: none |
| Applications： | cloning vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.7; HindIII--2.7; SalI--2.7; PstI--2.7; XbaI--2.7. The only difference between this and pUC12 is orientation of polylinker sites. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Messing J. New M13 vectors for cloning. Methods Enzymol. 101: 20-77, 1983. PubMed: 6310323 |
| Shipped： | freeze-dried |