微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pPAI-E5.2 |
|---|---|
| 商品货号： | TS158583 |
| Designations： | pPAI-E5.2 |
| GenBank Number： | J03764 |
| Species： | Homo sapiens, human |
| Depositors： | PJ Bosma |
| Applications： | There are 4 putative polyadenylation signals between nucleotides 12092 and 12141. There is also an AATAAT sequence at 11174 surrounded by an AT-rich region which could serve as a polyadenylation signal. |
| Vector： | Construct size (kb): 7.900000095367432 |
| Insert： | DNA: genomic Insert lengths(kb): 5.199999809265137 Gene product: plasminogen activator inhibitor, type I PAI1 |
| Insert Size (kb)： | 5.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Among the restriction sites in the insert are the following: PstI (4), BglI (2), BamHI (2), NcoI (1), AatII (1), BstEII (1), and XbaI (1). Restriction digests of the clone give the following sizes (kb): EcoRI--5.2, 2.7; PstI--3.3, 2.9, 0.98, 0.48, 0.24; BamHI--5.4, 1.4, 0.87; SalI--7.9; BglI--5.3, 1.3, 1.08. There are 4 putative polyadenylation signals between nucleotides 12092 and 12141. There is also an AATAAT sequence at 11174 surrounded by an AT-rich region which could serve as a polyadenylation signal. Insert begins in intron 7 and extends about 2.2 kb 3 to the last exon, exon 9. The insert contains multiple Alu repeats and two purine-rich sequences. |
| References： | Bosma PJ, et al. Human plasminogen activator inhibitor-1 gene. Promoter and structural gene nucleotide sequences. J. Biol. Chem. 263: 9129-9141, 1988. PubMed: 3132455 |