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| 产品名称： | pV-mos |
|---|---|
| 商品货号： | TS158637 |
| Designations： | pV-mos |
| GenBank Number： | J02266 |
| Species： | Moloney murine sarcoma virus, Moloney murine sarcoma virus |
| Depositors： | SA Aaronson, SR Tronick |
| Vector： | Construct size (kb): 5.199999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 0.8999999761581421 Gene product: Moloney murine sarcoma viral (v-mos) oncogene homolog MOS Alleles: B2, B1 |
| Insert Size (kb)： | 0.900 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The BglI site on the insert end was replaced by a HindIII linker before cloning. The insert is the BglI/HindIII fragment from 4116 to 5021 on the Mo-MSV map. The insert contains KpnI, HpaI, SstII, PstI (4), HpaII (5), DdeI (3), and HinfI (4) sites. The insert excludes v-mos helper viral junctions. |
| Classification： | Retroviridae, Gammaretrovirus, Mammalian virus group |
| References： | Reddy EP, et al. Complete nucleotide sequence and organization of the Moloney murine sarcoma virus genome. Science 214: 445-450, 1981. PubMed: 6170110 Tronick SR, et al. Molecular cloning of Moloney murine sarcoma virus: arrangement of virus-related sequences within the normal mouse genome. Proc. Natl. Acad. Sci. USA 76: 6314-6318, 1979. PubMed: 392518 |