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| 产品名称： | pLamB1L |
|---|---|
| 商品货号： | TS158647 |
| Designations： | pLamB1L |
| GenBank Number： | M15525 |
| Species： | Mus musculus, mouse |
| Depositors： | I Oberbaumer |
| Applications： | A HindIII/SphI digestion produces fragments of 1.630 kb (without tails) and 0.855 kb (with a GC tail). |
| Vector： | Construct size (kb): 5.349999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.654999971389771 Tissue: teratocarcinoma PYS-2/12 cell line Gene product: laminin, B1 polypeptide Lamb1 Alleles: a, l |
| Insert Size (kb)： | 2.655 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--2.8, 2.6; EcoRI--5.4; HindIII--2.9, 2.5; KpnI--5.4; SalI--5.4. The insert includes the following restriction sites (approximate kb from the 5 end): PvuII--0.12, 0.76, 1.38, 1.45, 2.23; ApaI--0.78; PstI--0.88, 1.40, 1.78, 2.3; SacI--0.89, 1.39; BglII--1.61; HindIII--2.48. A HindIII/SphI digestion produces fragments of 1.630 kb (without tails) and 0.855 kb (with a GC tail). The XbaI site at nt 4730 of the reported sequence does not cut. Extends from nt 2641 to 5296 of the reported sequence, encoding part of domains I and III and all of domains II and alpha. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Oberbaumer I. New pUC-derived expression vectors for rapid construction of cDNA libraries. Gene 49: 81-91, 1986. PubMed: 2436975 Sasaki M, et al. Sequence of the cDNA encoding the laminin B1 chain reveals a multidomain protein containing cysteine-rich repeats. Proc. Natl. Acad. Sci. USA 84: 935-939, 1987. PubMed: 3493487 Ilse Oberbaumer, personal communication |