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微信小章| 产品名称： | pT24-C3 |
|---|---|
| 商品货号： | TS158761 |
| Designations： | pT24-C3 |
| GenBank Number： | J00277 |
| Species： | Homo sapiens, human |
| Depositors： | M Barbacid |
| Vector： | Construct size (kb): 10.89999961853027 |
| Insert： | DNA: genomic Insert lengths(kb): 6.599999904632568 Tissue: bladder carcinoma T24 cell line Gene product: Harvey rat sarcoma viral (v-Ha-ras) oncogene homolog(Harvey rat sarcoma virus oncogene) HRAS Alleles: D1, D2 |
| Insert Size (kb)： | 6.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.6, 4.3; SacI--7.0, 3.0, 0.9. Shows transforming activity; insert contains 3 SacI sites (major fragments 3.0, 2.7, 0.85 kb), as well as XhoI, PvuII, XbaI, KpnI, SphI, ClaI, BglII sites; plasmid cut with SacI gives 7.0, 3.0, 0.85 kb fragments. This probe contains the transforming form of the HRAS1 gene mutated by a G to T transversion at the 12th codon; it also contains 3 and 5 flanking sequences. |
| References： | Iwahana H, et al. A new RFLP in intron 1 of the human c-Ha-ras1 gene and its close relationship with the variable tandem repeats in the region 3 to the gene. Oncogene 5: 1049-1053, 1990. PubMed: 1973828 Pulciani S, et al. Oncogenes in human tumor cell lines: Molecular cloning of a transforming gene from human bladder carcinoma cells. Proc. Natl. Acad. Sci. USA 79: 2845-2849, 1982. PubMed: 6953433 Santos E, et al. T24 human bladder carcinoma oncogene is an activated form of the normal human homologue of BALB- and Harvey-MSV transforming genes. Nature 298: 343-347, 1982. PubMed: 6283384 Reddy EP. Nucleotide sequence analysis of the T24 human bladder carcinoma oncogene. Science 220: 1061-1063, 1983. PubMed: 6844927 Pulciani S, et al. Transforming genes in human tumors. J. Cell. Biochem. 20: 51-61, 1982. PubMed: 6761348 |