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| 产品名称： | pCLVO12 |
|---|---|
| 商品货号： | TS158809 |
| Designations： | pCLVO12 |
| Species： | African cassava mosaic geminivirus |
| Depositors： | J Stanley |
| Applications： | The experimental host is Nicotiana benthamiana. |
| Vector： | Construct size (kb): 5.5 |
| Insert： | DNA: genomic Insert lengths(kb): 2.799999952316284 Gene product: genome, partial |
| Insert Size (kb)： | 2.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--5.4; BamHI--2.6 (doublet), 0.2; EcoRI--3.2, 1.4, 0.8; EcoRI/HindIII--2.7, 1.4, 0.8, 0.5. The experimental host is Nicotiana benthamiana. Contains the full-length DNA 1 linearized with EcoRV (+ orientation). The insert was derived from supercoiled DNA isolated from plants infected using clones pJS092 and pJS094. |
| Classification： | Geminivirus |
| References： | Stanley J. Infectivity of the cloned geminivirus genome requires sequences from both DNAs. Nature 305: 643-645, 1983. John Stanley, personal communication |