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微信小章| 产品名称： | pbPKC-alpha21 |
|---|---|
| 商品货号： | TS158821 |
| Designations： | pbPKC-alpha21 |
| GenBank Number： | M13973 |
| Species： | Bos taurus, cow |
| Depositors： | A Ullrich |
| Applications： | Hybridizes to human sequences and was used to map the human gene. |
| Vector： | Construct size (kb): 3.200000047683716 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.531000018119812 Tissue: brain Gene product: protein kinase C, alpha Pkca Alleles: b, s |
| Insert Size (kb)： | 0.531 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Contains the following restriction sites (with nucleotide position according to the published sequence): BamHI--608, HindIII--645, HindIII--975. Restriction digests of the clone give the following sizes (kb): BamHI--2.75, 0.45; PstI--3.2; XbaI--3.2; HindIII--2.85, 0.35; EcoRI--2.7, 0.5. Includes sequences from approximately nt 590 to nt 1120 of the published sequence, encoding the calcium-binding site and the ATP binding site. Coding sequences extend from nt 50 to nt 2066. Hybridizes to human sequences and was used to map the human gene. Detects human genomic EcoRI fragments of 4.7 and 3.8 kb and HindIII fragments of 4.2, 7.5, and 3.0 kb. Detects mRNA of 8.1, 3.8, and 3.5 kb in bovine brain and 8.6 kb in rat brain. |
| References： | Buchberg AM, et al. A comprehensive genetic map of murine chromosome 11 reveals extensive linkage conservation between mouse and human. Genetics 122: 153-161, 1989. PubMed: 2567264 Coussens L, et al. Multiple, distinct forms of bovine and human protein kinase C suggest diversity in cellular signaling pathways. Science 233: 859-866, 1986. PubMed: 3755548 Parker PJ, et al. The complete primary structure of protein kinase C--the major phorbol ester receptor. Science 233: 853-859, 1986. PubMed: 3755547 |