宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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lambdaHSA10 [KA27]

货号 TS158828
中文名称 null
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产品名称: lambdaHSA10 KA27
商品货号: TS158828
Designations: lambdaHSA10 KA27
GenBank Number:

M12523

Species: Homo sapiens, human
Depositors: A Dugaiczyk
Vector:
Construct size (kb): 49.70000076293945
Insert:
DNA: genomic
Insert lengths(kb): 20.29999923706055
Tissue: fetal liver
Gene product: albumin ALB
Alleles: E1, C1, C2, D1, F1, F2, A1, D2, E2, A2
Insert Size (kb): 20.300
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried bacteria-free lysate
Comments:
Restriction digests of the clone give the following sizes (kb): HindIII--22.0, 11.0, 7.1, 6.4, 5.8; EcoRI--19.4, 11.0, 9.5, 3.6, 2.2, 1.0; BamHI--26.0, 7.9, 6.1, 4.0, 2.1, 1.6;
PstI--12.5, 12.0, 5.0, 4.0, 2.7, 2.5, 2.4, 2.2, 2.0, 1.8, 1.2, 1.1, 0.6; SmaI--30.0, 7.1, 7.1.
The insert contains the following restriction sites (nt position according to the published sequence): EcoRI--2146, 5687, 7290, 7556, 18379; HindIII--5706, 5946, 12354, 18563; PstI--9072, 12586, 14849. Also contains 4 Alu repeats.
The 3 end overlaps the 5 end of lambdaHSA110 (ATCC 65732, 65733) by about 9 kb.
Corresponds approximately to nt 5 - 19016 of the published sequence, and thus includes all exons (primary transcript nt 1737 -18688 for one of two possible polyadenylation sites).
Also contains 4 Alu repeats.
References:

Minghetti PP, et al. Molecular structure of the human albumin gene is revealed by nucleotide sequence within q11-22 of chromosome 4. J. Biol. Chem. 261: 6747-6757, 1986. PubMed: 3009475

Hawkins JW, Dugaiczyk A. The human serum albumin gene: structure of a unique locus. Gene 19: 55-58, 1982. PubMed: 6292049