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| 产品名称： | Leishmania hertigi Herrer |
|---|---|
| 商品货号： | TS158964 |
| Strain Designations： | C117 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | prehensile-tailed porcupine, Coendou rothschildi, Sta Rita, Chorrera, Panama, 1972 |
| Product Format： | frozen |
| Type Strain： | no |
| Comments： | Promastigotes. species description lipids Cyclopropane fatty acid |
| Medium： | ATCC® Medium 811: Leishmania medium |
| Growth Conditions： | Temperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly. |
| Subcultivation： | Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly. |
| Name of Depositor： | NR Dollahon |
| Chain of Custody： | ATCC < |
| Year of Origin： | 1972 |
| References： | Herrer A. Leishmania hertigi sp. n., from the tropical porcupine, Coendou rothschildi Thomas. J. Parasitol. 57: 626-629, 1971. PubMed: 5090970 Beach DH, et al. Lipids of Leishmania promastigotes. J. Parasitol. 65: 201-216, 1979. PubMed: 448607 Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247 |