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| 产品名称： | c9433 |
|---|---|
| 商品货号： | TS159077 |
| Designations： | c9433 |
| GenBank Number： | U11582 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | L Riles, MV Olson |
| Vector： | Construct size (kb): 43.70000076293945 |
| Insert： | DNA: genomic Insert lengths(kb): 37.30300140380859 Gene product: sporulation recombination protein DUR3 |
| Insert Size (kb)： | 37.303 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--9.0, 7.0, 3.0, 2.7 (triplet), 2.4 (doublet), 2.1, 1.4, 1.2 (doublet), 1.1, 0.9, 0.8 and smaller bands. Reported to contain EcoRI/HindIII fragments of the following sizes (kb): 6.820 (vector), 3.015, 2.820, 2.721, 2.704, 2.572, 2.479, 2.142, 1.362, 1.244, 1.210, 1.106, 0.962, 0.841, 0.662, 0.639, 0.548, 0.501 (vector), 0.450, 0.435. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Corresponds to nt 45593-82896 of the sequence of chromosome VIII. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Johnston M, et al. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. Science 265: 2077-2082, 1994. PubMed: 8091229 Linda Riles, personal communication |