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| 产品名称： | pMK3 WM2276 |
|---|---|
| 商品货号： | TS159087 |
| Designations： | pMK3 WM2276 |
| Depositors： | W Messer |
| Applications： | contains easily purifiable cassette(s) for construction lacI - lacOP cassette |
| Vector： | Construct size (kb): 5.0 |
| Insert： | Target Gene: lacI - lacOP cassette |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglI--3.2, 1.45, 0.4; EcoRI--2.75, 2.15; XhoI--3.2, 1.8. Plasmid with an XhoI promoter cassette (1.777 kb) containing the wild type lac promoter. The vector component of this construct contains unknown sequence. This should be used only to excise the promoter-containing cassette. |
| References： | Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348 Walter Messer, personal communication |