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| 产品名称： | hHAOF9 |
|---|---|
| 商品货号： | TS159106 |
| Designations： | hHAOF9 |
| GenBank Number： | Z29481 |
| Species： | Homo sapiens, human |
| Depositors： | P Malherbe |
| Applications： | in another host, produces protein 3-hydroxyanthranilate 3,4-dioxygenase |
| Vector： | Construct size (kb): 6.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBC/CMV Intact vector size: 4.700 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: ClaI EcoRI BamHI XbaI SalI PstI HindIII Polylinker sites: ClaI EcoRI BamHI XbaI SalI PstI HindIII Host range: eukaryotic cells; Escherichia coli Features (with orientation and position when available): replicon: pMB1 promoter for expression: CMV, -> other: rat preproinsulin II (rI2) intron, -> terminator: SV40 polyadenylation signal, -> marker(s): ampR replicon: SV40 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: HAO-PEN Gene name: 3-hydroxyanthranilate 3,4-dioxygenase Contains complete coding sequence?: Y Tissue: hepatoma HepG2 cell line (ATCC HB8065) Type of DNA: cDNA Insert end: Modification: EcoRI linker Insert end: Modification: EcoRI linker Insert size (kb): 1.276 Cross references: DNA Seq. Acc.: Z29481 Nucleotides 1-1247 of the insert correspond to nucleotides 1-1247 of Z29481. Insert lengths(kb): 1.276000022888184 Tissue: hepatoma HepG2 cell line (ATCC HB8065) Gene product: 3-hydroxyanthranilate 3,4-dioxygenase HAO-PEN Target Gene: 3-hydroxyanthranilate 3,4-dioxygenase |
| Insert Size (kb)： | 1.276 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.7, 1.3; NcoI--4.0, 0.9, 0.5, 0.4, 0.3; PstI--5.4, 0.6. Insert contains the following restriction sites (approximate kb from the 5 end): NcoI--0.41, 0.91; PstI--0.62, 1.18; PvuII--0.21, 0.53, 0.73; SmaI--0.03; StuI--0.70; XhoI--0.95. Includes 40 nt of 5 untranslated sequence, the complete coding sequence and 346 nt of 3 untranslated sequence followed by a poly(A) tail |
| References： | Malherbe P, et al. Molecular cloning and functional expression of human 3-hydroxyanthranilic-acid dioxygenase. J. Biol. Chem. 269: 13792-13797, 1994. PubMed: 7514594 |